Research Specialist I

Req ID #:  110332

Bethesda, MD, US, 20892-0602

For 70 years, Charles River employees have worked together to assist in the discovery, development and safe manufacture of new drug therapies. When you join our family, you will have a significant impact on the health and well-being of people across the globe. Whether your background is in life sciences, finance, IT, sales or another area, your skills will play an important role in the work we perform. In return, we’ll help you build a career that you can feel passionate about.


IMPORTANT:   In order to be considered for this position, a resume/CV must be uploaded and submitted during the application process.  Please make sure work history and education are added correctly.   

Job Summary

We are seeking a Research Specialist 1 for our Insourcing Solutions site located in Bethesda, MD.


The following are responsibilities related to the Research Specialist 1:


•    Provide support for large scale mutagenesis screens, including generating fish lines, conducting relevant complementation testing and map-crossing, managing screen related data, and PCR-based fine mapping (marker analysis) to narrow down and/or isolate the mutant region/lesion. 
•    Perform technical procedures related to Aquatic species (e.g. obtaining blood and other samples, obtain zebrafish sperm for cryopreservation and in vitro fertilization, facilitate animal breeding and embryo collection, identify pathogens in zebrafish (P. neurophilia and Mycobacterium species) by PCR and DNA sequence analysis).
•    Assist post-doc and collaborators with research projects. Teach post-doc zebrafish reproductive physiology and care.
•    Assist with lab-based technical resource / instrument procurement and manage NICHD Program equipment usage.
•    Assist with all aspects of zebrafish related experimental design, including drafting SOPs and protocols for zebrafish related tasks; statistical analysis of data; generation of publication quality images, graphs and figures; scientific writing for publication.
•    Utilize a variety of online zebrafish genome databases for gene searches, candidate gene identification for mutation analysis, genomic DNA sequence analysis for PCR primers, mRNA / Protein sequence analysis, and CRISPR target analysis / generation. 
•    Technical and bench top duties including embryo cleaning, staging and fixation, mutant and transgenic identification and propagation, microinjection of DNA and RNA into zebrafish embryos, gDNA / RNA isolation, mRNA / cDNA synthesis, PCR, gel electrophoresis, in situ hybridization, molecular cloning, whole mount antibody staining, fluorescent microscopy,  western blotting, and CRISPR / Cas9 mutant zebrafish generation (all steps from gRNA target generation to locating genomic lesion in F2 mutants).
•    Help screen established mutant zebrafish lines using fluorescent microscopy, PCR / Restriction Enzyme Digest analysis, KASP genotyping assays on BioRad CFX, and Fluorescent PCR analysis on ABI 3130xl.
•    Maintain effective lines of communication with investigator regarding colony and support duties.
•    Actively interact with customers as required to solve customer requests, inquiries, and complaints and to provide technical assistance when applicable to job description.
•    Manage international and domestic shipment of zebrafish embryos and adults following Federal and NIH guidelines.
•    Comply with safety policies and procedures and MSDS guidelines, including those related to biohazards and radioactive substances. 
•    Troubleshoot aquatic support equipment and provides input and action on cause and remedy.



Job Qualifications

The following are minimum qualifications related to the Research Specialist 1 position:


Education: B.S. in Biological Sciences required.
Experience: At least 3 years in a laboratory research environment including basic molecular biology tasks. Aquatic species experience in a research environment is highly desired. 
Certification/Licensure:  Not applicable.



IMPORTANT: A resume is required to be considered for this position. If you have not uploaded your resume in your candidate profile, please return to upload field and attach your resume/CV. 

About Insourcing Solutions
Charles River Insourcing Solutions℠ can streamline research by delivering operational and cost efficiencies through the strategic insourcing of GMP, GLP and non-GLP research services from discovery through safety assessment. Insourcing Solutions℠ provides you with the ability to maintain control of your facility and expertise while leveraging available space.


About Charles River
Charles River is an early-stage contract research organization (CRO). We have built upon our foundation of laboratory animal medicine and science to develop a diverse portfolio of discovery and safety assessment services, both Good Laboratory Practice (GLP) and non-GLP, to support clients from target identification through preclinical development. Charles River also provides a suite of products and services to support our clients’ clinical laboratory testing needs and manufacturing activities. Utilizing this broad portfolio of products and services enables our clients to create a more flexible drug development model, which reduces their costs, enhances their productivity and effectiveness to increase speed to market.

With over 17,000 employees within 90 facilities in 20 countries around the globe, we are strategically positioned to coordinate worldwide resources and apply multidisciplinary perspectives in resolving our client’s unique challenges. Our client base includes global pharmaceutical companies, biotechnology companies, government agencies and hospitals and academic institutions around the world. And in 2019, revenue increased to $2.62 billion.

At Charles River, we are passionate about our role in improving the quality of people’s lives. Our mission, our excellent science and our strong sense of purpose guide us in all that we do, and we approach each day with the knowledge that our work helps to improve the health and well-being of many across the globe. We have proudly supported the development of ~85% of the drugs approved by the FDA in 2019.

Equal Employment Opportunity
Charles River Laboratories is an Equal Opportunity Employer - M/F/Disabled/Vet.

If you are interested in applying to Charles River Laboratories and need special assistance or an accommodation due to a disability to complete any forms or to otherwise participate in the resume submission process, please contact a member of our Human Resources team by sending an e-mail message to This contact is for accommodation requests for individuals with disabilities only and cannot be used to inquire about the status of applications.

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